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Quiles M, Boettger B, Pignatari ACC (2017) Update in Bloodstream Infection Diagnosis Using New Methods in Microbiology. Curr Treat Opt Inf Dis 9, 1-10.

Singh SP (2017) Nucleic acid-based methods for early detection of sepsis. Ann Card Anaesth 20, 112.

Lorenz MG , Lustig M, Linow M (2017) Fungal-grade reagents and materials for molecular analysis. In: Lion T (ed.), Human Fungal Pathogen Identification: Methods and Protocols, Methods in Molecular Biology, vol. 1508, p. 141-150. DOI 10.1007/978-1-4939-6515-1_4, © Springer Science+Business Media New York.

Lackner M, Lass-Flörl C (2017) Commercial molecular tests for fungal diagnosis from a practical point of view. In: Lion T (ed.), Human Fungal Pathogen Identification: Methods and Protocols, Methods in Molecular Biology, vol. 1508, p. 85-105. DOI 10.1007/978-1-4939-6515-1_4, © Springer Science+Business Media New York.

Nieman AE, Savelkoul PHM, Beishuizen A, Henrich B, Lamik B, MacKenzie CR, Kindgen-Milles D, Helmers A, Diaz C, Sakka SG Schade RP (2016) A prospective multicenter evaluation of direct molecular detection of blood stream infection from a clinical perspective. BMC Inf Dis  16, 314 DOI 10.1186/s12879-016-1646-4.

Berktold M, Mutschlechner W, Orth-Höller D (2016) Comparison of rapid hybridization-based pathogen identification and resistance evaluation in sepsis using the Verigene® device paired with “good old culture”. Wien Klin Wochenschr (2016). doi:10.1007/s00508-016-1057-y.

Eberhard J, Stumpp N, Winkel A, Schrimpf C, Bisdas T, Orzak P, Teebken OE, Haverich A, Stiesch M (2016) Streptococcus mitis and Gemella haemolysans were simultaneously found in atherosclerotic and oral plaques of elderly without periodontitis - a pilot study. Clin Oral Invest. DOI: 10.1007/s00784-016-1811-6.

Stappers MHT, Hagen F, Reimnitz P, Mouton JW, Meis JF, Gyssens IC (2016) Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection. Diagn Microbiol Inf Dis 85, 263 – 265.

Schwameis M, Auer J, Mitteregger D, Simonitsch-Klupp I, Ramharter M, Burgmann H (2016) Anaplasmataceae–specific PCR for diagnosis and therapeutic guidance for symptomatic neoehrlichiosis in immunocompetent host. Emerg Infect http://dx.doi.org/10.3201/eid2202.141762

Compain F, Bruneval P, Jarraud S, Perrot S, Aubert S, Napoly V, Ramahefasolo A, Mainardi JL, Podglajen I (2015) Chronic endocarditis due to Legionella anisa: a first case difficult to diagnose. New Microbes New Infect 8, 113–115.

Stappers MHT, Hagen F, Reimnitz P, Mouton JW, Meis JF, Gyssens IC (2015) Direct molecular versus culture-based assessment of Gram-positive cocci in biopsies of patients with major abscesses and diabetic foot infections. Eur J Clin Microbiol Inf Dis 34, 1885-1892.

Borde JP, Häcker GA, Guschl S, Serr A, Danner T, Hübner J, Burrack-Lange S, Lüdke G, Helwig P, Hauschild O, Kern WV (2015). Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study. Infection 43, 551-560.

Trinick RE, Bunni L, Thorburn K, Hackett AP, Dalzell M, McNamara PS (2015). An observational study examining the relationship between respiratory symptoms, airway inflammation and bacteriology in children with severe neurodisability. PLoS ONE 10(4): e0124627. doi:10.1371/journal.pone.0124627.

Stubljar D, Kopitar AN, Groselj-Grenc M, Suhadolc K, Fabjan T, Skvarc M (2015) Diagnostic accuracy of presepsin (sCD14-ST) to predict bacterial infection measured in cerebrospinal fluid in children with suspected bacterial meningitis/ventriculitis. J Clin Microbiol 53, 1239-1244.

Marsch G, Orszag P, Mashaqi B, Kuehn C, Haverich A (2015) Antibiotic therapy following polymerase chain reaction diagnosis of infective endocarditis: a single centre experience. Interac Cardiovasc Thorac Surg 20, 589-593.

Meyer T, Franke G, Polywka SKA, Lütgehetmann M, Gbadamosi J, Magnus T,  Aepfelbacher M (2014) Improved detection of bacterial central nervous system infections by use of a broad-range PCR assay J. Clin. Microbiol 52, 1751-1753.

Stubljar D, Skvarc M (2014) Expression of CD64 on neutrophils can be used to predict the severity of bloodstream infection before broad range 16S rRNA PCR. Folia Microbiologica 60, 111-118.

Rogina P, Skvarc M, Stubljar D, Kofol R, Kaasch A (2014) Diagnostic utility of broad range bacterial 16S rRNA gene PCR with degradation of human and free bacterial DNA in bloodstream infection is more sensitive than an in-house developed PCR without degradation of human and free bacterial DNA. Mediators of Inflammation, doi.org/10.1155/2014/108592.

Schukfeh N, Doerner JM, Heintschel von Heinegg E, Steinmann J, Metzelder ML, Kathemann S, Hoyer PF, Paul A, Gerner P (2014) Spectrum of pathogens in native liver, bile, and blood during pediatric liver transplantation. Ped Transplantation 18, 266–271.

Igressa A, Defosse J, Disqué C, Wappler F, Sakka SG (2014) Usefulness of broad-range PCR plus sequencing for the diagnosis of bacteremia due to a lung abscess. Int J Infect Dis, DOI: 10.1016/j.ijid.2014.04.018.

Loonen AJM, Wolffs PFG, Bruggeman CA, van der Brule AJC (2014) Developments for improved diagnosis of bacterial bloodstream infections. Eur J Clin Microbiol Inf Dis, doi: 10.1007/s10096-014-2153-4.

Valencia-Shelton F, Loeffelholz M (2014) Nonculture techniques for the detection of bacteremia and fungemia. Future Microbiol 9, 543-559.

Kothari A, Morgan M, Haake D (2014) Emerging technologies for rapid identification of bloodstream pathogens. Clin Inf Dis, doi: 10.1093/cid/ciu292.

Chang YT, Wang HC, Wang MC, Wu AB, Sung JM, Sun HS, Su IJ, Kan WC, Chien CC, Hwang JC, Wang HY, Tseng CC, Wu CJ (2014) Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis using multilocus PCR coupled with electrospray ionization mass spectrometry. J Clin Microbiol 52, 1217-1219.

Cayo R, Fehlberg LCC, Carvalhaes CG, Nicoletti AG, Gales AC (2014) Molecular diagnosis contributing for multi-drug resistant infection control. Curr Treatment Opt Inf Dis 6, 17-39.

Kühn C, Orszag P, Burgwitz K, Marsch G, Stumpp N, Stiesch M, Haverich A (2013) Microbial adhesion on membrane oxygenators in patients requiring extracorporeal life support detected by a universal rDNA PCR test. ASAIO Journal 59, 368-373.

Wu CJ, Chen YP, Wang HC, Su IJ, Ko WC, Chen JS, Cheng CN, Lee NY, Sun HS, Chi CY, Chen TY (2013) Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry. Diagn Microbiol Infect Dis 78, 141-143.

Orszag P, Disqué C, Keim S, Lorenz MG, Wiesner O, Hadem J, Stiesch M,  Haverich A, Kühn C (2013) Monitoring of patients supported by extracorporeal membrane oxygenation for systemic infections by broad-range rRNA gene PCR amplification and sequence analysis. J Clin Microbiol 52, 307-311.

Haag H, Locher F, Nolte O (2013) Molecular diagnosis of microbial aetiologies using SepsiTest™ in the daily routine of a diagnostic laboratory. Diag Microbiol Inf Dis 76, 413-418.

Shin JH, Sharaan M, Wu J, Cui M, Zhang DY, Tang YW (2013) Molecular bacteriology mycobacteriology. In: Cheng L, Zhang DY, Eble JN (eds.), Molecular Genetic Pathology. Springer Science and Business Media, New York. doi 10.1007/978-1-4614-4800-6_26.

Skvarc M, Stubljar D, Rogina P, Kaasch AJ (2013) Non-culture based methods to diagnose bloodstream infections: does it work? Eur J Microbiol Immunol 3, 97–104.

Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng CP, Lee CC (2013) Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis – a systemic review and meta-analysis. PLOS One 8, e62323. doi:10.1371/journal.pone.0062323.

Lebovitz EE, Burbelo PD (2013) Commercial multiplex technologies for the microbiological diagnosis of sepsis. Mol Diagn Ther 17, 221-231.

Schreiber J, Nierhaus A, Braune SA, de Heer G, Kluge S (2013) Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients. Med Klin Intensivmed Notfmed 108, 311-318.

Kabir MA, Ahmad Z (2013) Candida infections and their prevention. ISRN Preventive Medicine, vol. 2013, Article ID 763628, 13 pages, 2013. doi:10.5402/2013/763628.

Grif K, Heller W., Prodinger M, Lechleitner K, Lass-Flörl C, and Orth D (2012) Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis. J Clin Microbiol 50, 2250-2254

Kühn C, Disqué C, Mühl H, Orszag P, Stiesch M, Haverich A (2011) Evaluation of commercial universal rRNA gene PCR plus sequencing tests for identification of bacteria and fungi associated with infectious endocarditis. J Clin Microbiol 49, 2919-2923.

Schaub N, Frei R, Müller C (2011) Addressing unmet clinical needs in the early diagnosis of sepsis. Swiss Med Wkly. 141 (in press); doi:10.4414/smw.2011.13244

Pletz MW, Wellinghausen N, Welte T (2011) Will polymerase chain reaction (PCR)-based diagnostics improve outcome in septic patients? A clinical view. Intensive Care Med 37, 1069–1076.

Oliva A, Belvisi V, Iannetta M, Andreoni C, Mascellino MT, Miriam Lichtner M, Vullo V, Mastroianni CM (2010) Pacemaker lead endocarditis due to multidrug-resistant Corynebacterium striatum detected with sonication of the device. J Clin Microbiol 48, 4669-4671.

Nieman AE, Rozemeijer W, de Jong E, Beishuizen B, van Agtmael MA, Savelkoul PHM, Schade RP (2010) Molecular detection of bacterial bloodstream infections: validation of the SepsiTest™ assay. Ned Tijdschr Med Microbiol 18 Supplement, Abstract P048, 109.

von Loewenich FD, Geißdörfer W, Disqué C, Matten J, Schett G, Sakka SG, Bogdan C (2010) Detection of "Candidatus Neoehrlichia mikurensis" in two patients with severe febrile illnesses: evidence for a european sequence variant. J Clin Microbiol 48, 2630-2635.

Disqué C, Sakka SG, Wellinghausen N (2010) Klinische Evaluation von SepsiTest™ zum universellen PCR-Nachweis von bakteriellen Erregern in Vollblut. Der Mikrobiologe Heft 1, Februar 2010, 13-16.

Sakka SG, Kochem AJ, Disqué C, Wellinghausen N (2009)
Blood infection diagnosis by 16S rDNA broad-spectrum polymerase chain reaction: the relationship between antibiotic treatment and bacterial DNA load. Anesthesia Analgesia 109, 1707-1708.

Wellinghausen N, Kochem AJ, Disqué C, Mühl H, Gebert S, Winter J, Matten J, Sakka SG (2009) Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis. J Clin Microbiol 47, 2759-2765.